Properties of shaker-type potassium channels in higher plants
Potassium (K(+)), the most abundant cation in biological organisms, plays a crucial role in the survival and development of plant cells, modulation of basic mechanisms such as enzyme activity, electrical membrane potentials, plant turgor and cellular homeostasis. Due to the absence of a Na(+)/K(+) e …
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Biophysical and Molecular Mechanisms of Shaker Potassium …
The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus …
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Initial steps in the opening of a Shaker potassium channel
We examined the effects of La 3+ on voltage-gated Shaker K + channels. Addition of the trivalent cation La 3+ (50 μM) extracellularly markedly prolongs the lag that precedes …
Which is better for topsoil, a Shaker or Trommel Screen?
The question of shaker or trommel for topsoil dates back many years, but over the past two decades the shaker screen category has expanded and trommel screens have changed, so let's see where they all stand now. SHAKER SCREENS. There are three types of shaker screens typically found on portable equipment. Sand and Gravel Screeners
Activation of Shaker Potassium Channels
This second of three papers, in which we functionally characterize activation gating in Shaker potassium channels, focuses on the properties of a mutant channel (called V2), in which the leucine at position 382 (in the Shaker B sequence) is mutated to valine. The general properties of V2's ionic and gating currents are consistent with changes in late gating …
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Charge-voltage curves of Shaker potassium channel are not …
For instance, Q-V curves of Shaker potassium channel obtained by hyperpolarizing from 0 mV is left-shifted compared to those obtained by depolarizing from a holding potential of −80 mV. This hysteresis in Q-V curves is a common feature of channels in the VGIC superfamily and raises …
Cell-free expression of functional Shaker potassium channels
Here we combine cell-free protein translation, microsomal membrane processing12–14 of nascent channel proteins, and reconstitution of newly synthesized ion channels into planar lipid bilayers15 ...
Activation of Shaker Potassium Channels
This second of three papers, in which we functionally characterize activation gating in Shaker potassium channels, focuses on the properties of a mutant channel (called V2), in which the leucine at position 382 (in the Shaker B sequence) is mutated to valine. The general properties of V2's ionic and gating currents are consistent with changes ...
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U-type inactivation of Kv3.1 and Shaker potassium channels.
The time and voltage dependence of recovery from inactivation reveals two distinct components for Shaker. Strong depolarizations favor inactivation that is reduced by K(o)(+) or by partial …
Structure of the Shaker Kv channel and …
Voltage-activated potassium (Kv) channels open and close in response to changes in membrane voltage, serving critical functions in electrical signaling in neurons and muscle, neurotransmitter and hormone secretion, cell …
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Activation of Shaker Potassium Channels
In this paper, we extend the kinetic analysis of the Shaker channel to the effects of the V2 mutation. This characterization of V2 serves two purposes. First, it serves to further …
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Low Potassium (Hypokalemia): Symptoms and Causes
Knowing the symptoms of low potassium, or hypokalemia, may allow you to recognize a potassium deficiency if you develop one. Potassium is a mineral needed in small amounts for cell, nerve, and muscle function. Under normal circumstances, your body will automatically control potassium levels in your blood.
Activation of Shaker Potassium Channels
The conformational changes associated with activation gating in Shaker potassium channels are functionally characterized in patch-clamp recordings made from Xenopus laevis oocytes expressing Shaker channels with fast inactivation removed, forming the basis for constraining a detailed gating model to be described in a subsequent paper of this series. The …
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Identification of Shaker Potassium Channel Family Members …
K + channels of the Shaker family have been shown to play crucial roles in K + uptake and transport. Cotton (Gossypium hirsutum) is an important cash crop.In this study, the 24 Shaker family genes were identified in cotton. Phylogenetic analysis suggests that they were assigned to five clusters. Additionally, their chromosomal location, conserved motifs and gene …
Biophysical and Molecular Mechanisms of Shaker …
The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed mutagene-sis and single …
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Initial steps in the opening of a Shaker potassium channel
The resting and activated conformations of the K V 1.2–2.1 voltage-sensing domain. (A) Experimentally determined activated conformation (Protein Data Bank ID code 2R9R).Note that R1 in Kv1.2–2.1 is glutamine, whereas R1 in Shaker is arginine. (B) Hypothetical resting/closed conformation.The yellow sphere represents the Ca 2+ in the hypothetical …
Biophysical and Molecular Mechanisms of Shaker Potassium …
The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed …
Hydropathic analysis and comparison of KcsA and Shaker …
We calculated and compared the hydropathic profiles of two K (+) channels, KcsA and Kv1.2 (the latter a member of the Shaker family), at their pore-forming domain.